Fig 1: The histological architecture between the tumor and the third ventricular floor. In (A–F), immunofluorescent staining shows a layer of gliosis (GFAP positive) with various thickness between the tumor and the neural tissue of the third ventricle floor. The neural tissue of the third ventricle floor/hypothalamus (NF and OXT positive) can be observed on the outer border of the gliosis. The ependymal cells of the third ventricle (white pentagram) could be found in some samples, which indicated the extraventricular topography of CP.
Fig 2: Immunofluorescence images showing dsRed-labeled afferent neuron with AVP and Oxt in the bilateral PVH. Immunofluorescence images showing that some dsRed-labeled neurons were co-localized with AVP neurons in the ipsilateral (A) and the contralateral PVH (B). Immunofluorescence images showing that dsRed-labeled neurons rarely co-localized with Oxt neurons in the ipsilateral (C) and the contralateral PVH (D). Scale bar, 50 µm. Data were obtained from four independent experiments.
Fig 3: Transcriptomic survey of PtenY68H/+ cortex identifies broad changes in expression, affecting PTEN signaling and neurological processes, including oxytocin overexpression. a Volcano plot highlighting genes showing highly significant changes in differential expression (DE) in red (P < 0.0001; NWT = 6; NY68H = 5). b Heatmap of DE genes (threshold: P < 0.001) clustered by column (i.e., by expression pattern within a gene). c Top “Canonical Pathways” identified by Ingenuity Pathway Analysis (IPA) from DE gene list input. d Top identified IPA network from input DE gene list. The network is organized hierarchically. Red = overexpression; green = underexpression; solid line = direct relationship; hashed line = indirect relationship; arrow direction = direction of relationship. e STRING analysis of subnetwork of DE genes displaying the relationship between overexpressed Oxt and other DE genes (i.e., interactome). Node size trends with degree connectivity. Node color trends with betweenness centrality (i.e., cooler colors indicated higher betweenness centrality). Thickness of edge trends with the confidence in the biological relationship. f An oxytocin regulatory network constructed using IPA’s Grow and Molecule Activity Predictor (MAP) tools. Blue = predicted inactivation; orange = predicted activation; solid line = direct relationship; hashed line = indirect relationship; arrow direction = direction of relationship
Fig 4: Overexpression of oxytocin in paraventricular nucleus (PVN) of the hypothalamus of six-week-old Pten+/+ and PtenY68H/+ mice. a Representative immunofluorescence staining of six-week-old Pten+/+ and PtenWT/Y68H PVN for Oxt (green) and DAPI (blue). N = 3. Magnification = 10X. Scale bar = 166 µm. b Quantification of the integrated density of Oxt stain per biological replicate, finding a significant increase in Oxt in the PtenY68H/+ PVN compared to Pten+/+ PVN (P = 0.032). c Western analysis for Oxt expression in of six-week-old Pten+/+ and PtenY68H/+ hypothalamus (N = 5). d Quantification of Western in panel c (P = 0.0079). P value key: *P < 0.05, **P < 0.01
Fig 5: Immunofluorescence images showing dsRed-labeled afferent neurons with AVP and Oxt in the bilateral SON. Immunofluorescence images showing that some dsRed-labeled neurons were co-localized with AVP neurons in the ipsilateral (A) and the contralateral SON (B). Immunofluorescence images showing that dsRed-labeled neurons rarely co-localized with Oxt neurons in the ipsilateral (C) and the contralateral SON (D). Scale bar, 50 µm. Data were obtained from four independent experiments.
Supplier Page from Abcam for Anti-Oxytocin antibody [EPR20973]